HUMORAL IMMUNE RESPONSES IN PIGS IMMUNIZED WITH TOXOPLASMA GONDII RECOMBINANT PROTEIN (RROP2) AND ISCOMATRIX ADJUVANT
Autor(es): Ivo Alexandre Leme da Cunha, Alessandra Taroda, Luiz Daniel de Barros, Dauton Luiz Zulpo, Jonatas Campos de Almeida, Ana Sue Sammi, Ana Carolina Miura, Italmar Teodorico Navarro, João Luis Garcia
HUMORAL IMMUNE RESPONSES IN PIGS IMMUNIZED WITH TOXOPLASMA GONDII RECOMBINANT PROTEIN (RROP2) AND ISCOMATRIX ADJUVANT
» Área de pesquisa: PROTOZOOLOGIA
» Instituição: Universidade Estadual de Londrina
» Agência de fomento e patrocinadores: CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico.
CAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível superior.
Toxoplasma gondii is an obligate intracellular parasite that infects a wide range of hosts including human beings and pork is considered the most important route of transmission. Recent decades have witnessed a great increasing in the number of technologies and immunological research to produce new vaccines. This study aims to evaluate the humoral immunity in pigs immunized intranasally and intramusculary with T. gondii recombinant protein (rROP2) plus Iscomatrix. We used 12 crossbred pigs, divided into three groups, G1 (n = 4) received recombinant proteins (rROP2; 200 µg) associated with Iscomatrix (50 µg), G2 (n = 4), and G3 (n = 4) which received Iscomatrix (50 µg) and PBS, respectively. All treatments were administered by nasal route (days 0, 14, 28, 42, 56 and 72) and by intramuscular route (days 86, 96 and 100). On day 111, all animals were challenged with 1,5x104 infective T. gondii oocysts of the VEG strain and at day 145 all animals were slaughtered. Clinical signs and temperature were analyzed before and after challenge. The humoral response (IgG, IgM AND IgA) was evaluated weekly using indirect immunoenzymatic test (ELISA). The presence of cysts in the muscles (heart, masseter, tongue and diaphragm) of pigs was analyzed by bioassay in mice. The only clinical sign was fever observed in pigs from the 6th to 8th day after challenge. All animals of G1 developed a high IgG and IgA antibody titers, ten days after the first dose of intramuscular vaccine, persisting until slaughter. The animals from G2 and G3 developed a low response 15 days after of challenge with oocysts. All animals developed a low level of IgM antibodies. In conclusion, the animals were stimulated in part in humoral immune response.
