ANAIS 2014

TARGETED SELECTIVE TREATMENTS WITH EUCALYPTUS STAIGERIANA NANOEMULSION AS PHYTOTHERAPIC ALTERNATIVE TO CONTROL SHEEP HEMOCHOSIS Autor(es): Wesley Lyeverton Correia Ribeiro, Jessica Maria Leite dos Santos, Weibson Paz Pinheiro André, Jose Vilemar de Araújo Filho, Ana Lourdes Camurça Fernandes Vasconcelos, Iara Tersia Freitas Macedo, Haroldo Cesar Beserra de Paula, Claudia Maria Leal Bevilaqua

TARGETED SELECTIVE TREATMENTS WITH EUCALYPTUS STAIGERIANA NANOEMULSION AS PHYTOTHERAPIC ALTERNATIVE TO CONTROL SHEEP HEMOCHOSIS

» Á�rea de pesquisa: HELMINTOLOGIA

» Instituição: UNIVERSIDADE ESTADUAL DO CEARÁ

» Agência de fomento e patrocinadores: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Capes)

Phytotherapy has been described as an alternative method for the control of gastrointestinal nematodes in small ruminants. The nanoencapsulation in biopolymer matrices can optimize the nematicidal effect of essential oils. The aim of this study was to evaluate Eucalyptus staigeriana nanoemulsion (EsNano) as an alternative to the control of sheep hemochosis. A mixture of E. staigeriana essential oil and Tween 80 at the ratio of 4:1 was added to a solution of 1% chitosan (w/w) under stirring at 18.000 rpm for 5 min on a mechanic stirrer. The size and distribution of nanoparticles were determined using a beam of red light with a wavelength of 633 nm. Male and female sheep were identified and divided into two groups (n=90) based on the epg, age, sex and body weight. G1 (positive control) were treated with 7.5 mg.kg-1 levamisole and G2 250 mg.kg-1 EsNano. A total of nine visits (between April and October 2013) were made to the commercial farm at intervals of 17 days. The ocular mucous membrane were examined and classified according to FAMACHA© and the animals scored 3, 4 or 5 were treated. The results were submitted to ANOVA and compared by Tukey test (p <0.05). The EsEO doping in the nanoemulsion was 36.36% (w/w). The average particle size was 274.3 nm with bimodal distribution and polydisperse. The average frequency of treated animals during visits were 8.65 and 8.92% for G1 and G2, respectively, without significant difference. On average, 2,43 and 2.64% of the animals with 3, 4 or 5 FAMACHA© for G1 and G2, respectively, maintained the score in the subsequent visit. EsNano showed the same efficacy as levamisole, anthelmintic previously tested in this population of H. contortus and demonstrated 97% average efficiency in faecal egg count reduction test. In this sense, EsNano characterized as alternative to control of H. contortus.