MOLECULAR AND MICROSCOPIC DETECTION OF HAEMOSPORIDIANS PARASITES IN ZENAIDA AURICULATA TRAP-CAPTURED FROM LONDRINA, PARANÁ
Autor(es): ALESSANDRA TARODA, BEATRIZ DE SOUSA LIMA NINO, LUIZ DANIEL DE BARROS, IVO ALEXANDRE LEME DA CUNHA, DAUTON LUIZ ZULPO, JOÃO PEDRO SASSE, MERCIA DE SEIXAS, JOELENI ROSA DOS SANTOS, THAIS AGOSTINHO MARTINS, MILTON HISSASHI YAMAMURA, ODILON VIDOTTO, JOÃO LUIS GARCIA
MOLECULAR AND MICROSCOPIC DETECTION OF HAEMOSPORIDIANS PARASITES IN ZENAIDA AURICULATA TRAP-CAPTURED FROM LONDRINA, PARANá
» Área de pesquisa: PROTOZOOLOGIA
» Instituição: Universidade Estadual de Londrina-PR
» Agência de fomento e patrocinadores: CAPES NO. 1345/11
Zenaida auriculata is endemic to an area ranging from Antilles to Tierra del Fuego, passing through Brazil. This dove is popularly known as the eared dove and is found in fields, farms and urban areas. The aim of this work was to identify the presence of hemoparasites in Zenaida auriculata, trap-captured from Londrina, Paraná. The blood samples were collected from the ulnar vein of 10 pigeons in EDTA tubes. Blood smears were made immediately after the collection. The slides were air dried at room temperature, fixed in methanol and stained by Giemsa method. Blood samples were also stored in EDTA tubes and frozen (-20°C). DNA were extracted using Kit Wizard® Genomic DNA Purification Kit (Promega MA, EUA), following the manufacture’s guidelines. For the Nested-PCR, the primers used were HaemNF1/HaemNR3 (first reaction) and HaemF/HaemR2 (second reaction), to identify the mitochondrial cytochrome b gene sequences. All the samples showed the presence of haemosporidians in both blood smears and Nested-PCR. The Haemoproteus sp. macrogametocytes and microgametocytes were detected in 10 (100%) blood smears. Nested-PCR showed the 525bp of Haemoproteus/Plasmodium genes. It was not possible to differentiate them because the primers chosen amplify a portion of the cytochrome b gene of the mitochondria that belongs to both genera. To differentiate the genera, the sequencing must be conducted. Haemoproteus sp. is considered apathogenic, but Plasmodium sp. can cause avian malariae. These haemosporidian can infect other species of birds and cause death. The sequencing could be important to identify the species of genera Haemoproteus and genera Plasmodium.
