NEMATOPHAGOUS FUNGUS POCHONIA CHLAMYDOSPORIA AGAINST E. PARAENSEI EGGS IN SUPPLEMENTED CULTURE MEDIA
Autor(es): João Victor Facchini Rodrigues, Rosane Teixeira Lelis, Fabio Ribeiro Braga, Lorendane Millena de Carvalho, Mariana Costa Fausto, Samuel Galvão de freitas, Alessandra Teixeira de Paula, Arnaldo Maldonado Junior, Juberlan Silva Garcia, Jackson Victor de Araújo
NEMATOPHAGOUS FUNGUS POCHONIA CHLAMYDOSPORIA AGAINST E. PARAENSEI EGGS IN SUPPLEMENTED CULTURE MEDIA
» Área de pesquisa: HELMINTOLOGIA
» Instituição: Universidade Federal de Viçosa
» Agência de fomento e patrocinadores: Conselho Nacional de Desenvolvimento Científico e Tecnológico - CNPq
Fundação de Amparo à Pesquisa do Estado de Minas Gerais - FAPEMIG
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES
The echinostomas have a broad geographic distribution and can infect wildlife, domestic animals, as well as humans, and so represent an important infective agent from medical and veterinary standpoints. Echinostoma paraensei presents a biological cycle composed of seven stages of evolution and the infection can occur by the ingestion of molluscs, fish, or raw/undercooked meat of amphibians containing metacercariae. One method of biological control employs ovicidal nematophagous fungi that can act directly on the free-living stage of most helminths. To evaluate ovicidal action of the species Pochonia chlamydosporia (isolate VC4) in supplemented culture media, 1000 E. paraensei eggs were placed in Petri dishes with different supplemented agar culture media and with the isolate VC4, as follows: (1) 2% chitin–agar (2% CA); (2) 2% cornmeal–agar (2% CMA); (3) 2% water–agar (2% WA); and (4) 2% starch–agar (2% SSA). Fungi were not used in the control group. Six repetitions were performed for each group. After 25 days 100 eggs were removed from each plate containing the isolate VC4 and from the control plate (without fungus). The ovicidal effect (destruction of eggs) of the isolate VC4 in supplemented agar-based culture media (chitin, corn and starch) were evaluated. The culture medium 2% SSA showed the best results for the destruction of eggs at the end of 25 days of interaction, with a percentage of 46.6%; on the other hand, the medium supplemented with corn (2% CMA) demonstrated an average destruction of 45.0%. The medium supplemented with chitin (2% CA) showed a relatively lower value (40.9%) when compared with 2% SSA and 2% CMA media, but the ovicidal activity of P. chlamydosporia was better in that medium, compared with the 2% WA medium (38.2%), demonstrating, possibly, a positive interference of chitin in the activity of this fungus.
