IMMUNIZATION OF WISTAR FEMALE RATS WITH 255-GY-IRRADIATED TOXOPLASMA GONDII: PREVENTING PARASITE LOAD AND MATERNOFOETAL TRANSMISSION
Autor(es): Lucilene Granuzzio Camossi, Felipe Fornazari, Virgínia Bodelão Richini Pereira, Rodrigo Costa da Silva, Daniel Fontana Ferreira Cardia, Helio Langoni
IMMUNIZATION OF WISTAR FEMALE RATS WITH 255-GY-IRRADIATED TOXOPLASMA GONDII: PREVENTING PARASITE LOAD AND MATERNOFOETAL TRANSMISSION
» Área de pesquisa: PROTOZOOLOGIA
» Instituição: Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista- UNESP, Campus de Botucatu, Departamento de Higiene Veterinária e Saúde Pública, Botucatu-SP, Brazil.
» Agência de fomento e patrocinadores: FAPESP 2010/02141-2
Toxoplasmosis, caused by an obligate intracellular protozoan parasite, Toxoplasma gondii, is an worldwide parasitic disease, with significant importance for animal production and considerable impact to the public health. This study was aimed to evaluate the dynamic of the distribution of Toxoplasma gondii in tissues of female wistar rats and their puppies tissues, after the immunization by oral rote with three dose of 1mL containing 1x107 irradiaded tachyzoites. One week after pregnancy confirmation, rats was challenged by gavage with bradyzoites, oocysts or tachyzoites of T. gondii. Forty-eight pregnant rats were grouped as follow: immunized and challenged with 102 bradyzoites.mL-1 (BZ*); non-immunized and challenged with 102 bradyzoites.mL-1 (BZ); immunized and challenged with 102 oocysts.mL-1 (OC*); non-immunized and challenged with 102 oocysts.mL-1 (OC); immunized and challenged with 102 tachyzoites.mL-1 (TZ*); non-immunized and challenged with 102 tachyzoites.mL-1 (TZ); only immunized (I); control group (C). After parturition the rats were sacrificed and the tissues were researched for the DNA of T. gondii by polymerase chain reaction (PCR) and the parasite load determined by the quantitative PCR (qPCR). It was verified that the immunization with irradiated tachyzoites of T. gondii induced the reduction of parasitic load in most organs analyzed, although not prevent the establishment of infection with the parasite. And also, the immunization showed a favorable effect on the birth rate and litter size.
