PHYLOGENETIC ANALYSIS OF APLASMATACEAE ORGANISMS INFECTING DOGS FROM RIO DE JANEIRO STATE
Autor(es): Ananda Muller Preira, Helena Keiko Thoma, Pedro Bittencourt Velho, Nádia Regina Pereira Almosny
PHYLOGENETIC ANALYSIS OF APLASMATACEAE ORGANISMS INFECTING DOGS FROM RIO DE JANEIRO STATE
» Área de pesquisa: DOENÇAS VETORIAIS
» Instituição: Universidad Austral de Chile
» Agência de fomento e patrocinadores: FAPERJ, CNPQ
This work intended assess the genetic diversity of Anaplasmataceae organisms naturally infecting dogs from Rio de Janeiro state through 16s rRNA gene sequencing. 30 Anaplasmataceae positive samples were obtained from naturally infected dogs diagnosed by PCR from six geopolitical areas of Rio de Janeiro: Metropolitana, Norte Fluminense, Centro sul Fluminense, Serrana, Noroeste Fluminense and Médio Paraíba. 16S rRNA genes were partially (n=30) (Protocol I: primers EHR16SD-EHR16SR) and completely (n=12) sequenced (Protocol II: primers A17-817 and 750-EC3). Resultant sequences from partial and nearly entire 16s rRNA gene were aligned with reference Anaplasmataceae sequences from GenBank, using the neighbor-joining method (MEGA). Protocol I Alignments allowed to identify 29 species infecting dogs, being 7 A. platys, 22 E. canis and one sample described as Anaplasma sp. Two Protocol I separate trees (E. canis tree (n=22) and Anaplasma sp. tree (n=8)) and one Protocol II tree (n=12) were constructed. The cladogram analysis of partial sequences (Protocol I) was performed carefully, sticking to the fact that the size of the aligned sequences may have limited comparison since only 348pb were available. Phylogenetics showed high diversity on some E. canis (0%-1.7%) and Anaplasma sp. (0-0.9%) partial sequences (348pb) and on A. platys complete sequence (1280pb) (n=7) from Rio de Janeiro state, when compared to geographical dispersed samples. These Rio de Janeiro samples were grouped on a single clade in phylogenetic tree, pointing to a differentiated evolution of these agents in the state. This divergence may have occurred either by an earlier insertion of the pathogen in Rio de Janeiro than that reported in other geographic regions or by an evolution and selective parasite pressure related to vertebrate and invertebrate hosts. E. canis nearly complete sequences (1280pb) (n=5) from Rio de Janeiro were highly conserved (99.6%-100%). Further studies with more divergent genes are required for confirm our results.
