ACTION OF THE CRUDE EXTRACT OF POCHONIA CHLAMYDOSPORIA AGAINST ECHINOSTOMA PARAENSEI EGGS
Autor(es): LORENDANE MILLENA DE CARVALHO, ROSANE TEIXEIRA LELIS, FABIO RIBEIRO BRAGA, MARIANA COSTA FAUSTO, JOÃO VICTOR FACCHINI RODRIGUES, ALESSANDRA TEIXEIRA DE PAULA, FILIPPE ELIAS FREITAS SOARES, JULIANA MILANI ARAÚJO, KLÉCILA REJANE PORTES REIS, JACKSON VICTOR DE ARAÚJO
ACTION OF THE CRUDE EXTRACT OF POCHONIA CHLAMYDOSPORIA AGAINST ECHINOSTOMA PARAENSEI EGGS
» Área de pesquisa: HELMINTOLOGIA
» Instituição: UNIVERSIDADE FEDERAL DE VIÇOSA
» Agência de fomento e patrocinadores: CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
FAPEMIG - Fundação de Amparo à Pesquisa do estado de Minas Gerais
CAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
Echinostoma paraensei is a trematode that causes echinostomiasis in humans. The infection can occur by the ingestion of mollusks, fish, or raw/undercooked meat of amphibians containing metacercariae. The ovicidal capacity of the crude enzymatic extract of P. chlamydosporia (VC4) on E. paraensei eggs was evaluated. To produce the crude extract, fungal mycelia were obtained by transferring culture disks (approximately 5 mm diameter) of fungal isolates kept in 2% CMA to Erlenmeyer flasks (250 ml) containing 50 ml of liquid medium, that was composed of: glucose (10 g/l); casein (18.409 g/l); K2HPO4 (5.0 g/l); MgSO4 (0.10 g/l); ZnSO4 (0.0050 g/l); FeSO4 (0.001 g/l); and CuSO4 (0.50 mg/l). The fungal inoculum was grown in the Erlenmeyer flasks under agitation of 120 revolutions per min at pH 9.0. After 6 days the supernatant fraction was collected and filtered on Whatman filter paper nº 1 at 4°C. A total of 1000 E. paraensei eggs were poured into Petri dishes of 4.5 cm in diameter with 5 ml of crude extract of P. chlamydosporia, constituting the treated group. The control group consisted of 1000 eggs in 10 ml of distilled water in Petri dishes. Petri dishes of the treated and control groups were incubated at 26°C and each treatment consisted of six replicates. After 7 days, the total number of E. paraensei eggs present in each Petri dish from the treated and control groups was calculated. The crude extract (extracellular enzymes) of VC4 was shown to be effective in destroying E. paraensei eggs at the end of 7 days, with a percentage reduction of 53% compared with the control group.
