INTERACTION OF THE HELMINTOPHAGOUS FUNGUS POCHONIA CHLAMYDOSPORIA AND PARASCARIS EQUORUM EGGS IN DIFFERENT CULTURE MEDIA
Autor(es): LORENDANE MILLENA DE CARVALHO, FABIO RIBEIRO BRAGA, MARIANA COSTA FAUSTO, JOÃO VICTOR FACCHINI RODRIGUES, MARISA CAIXETA VALADÃO, THAÍS DE OLIVEIRA, BRUNO MELO GASPAR, KLÉCILA REJANEPORTES REIS, JANVIEVY MARINÊS ANDRADE, JACKSON VICTOR DE ARAÚJO
INTERACTION OF THE HELMINTOPHAGOUS FUNGUS POCHONIA CHLAMYDOSPORIA AND PARASCARIS EQUORUM EGGS IN DIFFERENT CULTURE MEDIA
» Área de pesquisa: HELMINTOLOGIA
» Instituição: UNIVERSIDADE FEDERAL DE VIÇOSA
» Agência de fomento e patrocinadores: CNPq - Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico
FAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
CAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
Infection by Parascaris equorum is common in young horses and donkeys. It may cause cramps, present as moderate enteritis, interfere with the development of animals, impeding body growth, and is fatal in some cases. Research involving the use of helmintophagous fungi in the biological control of parasites of interest to veterinarians has occurred over recent years, with promising results. The infection of Parascaris equorum eggs by the fungus Pochonia chlamydosporia (isolates VC1 and VC4) was evaluated. Six groups were formed for each isolate, with six different culture media: 2% water–agar (2% WA); agar–chitin (AC); YPSSA (yeast extract, K2HPO4, MgSO4•7H2O, soluble starch); AELA extract (starch + water+ agar); 2% corn-meal–agar (2% CMA); and 2% potato dextrose–agar (2% PDA). A total of 1000 eggs of P. equorum were transferred to each plate containing isolates grown for a period of 7 days (treatment group). 1000 eggs were added to each plate without fungus (control group). The plates were kept in an environmental chamber at 25 °C in the dark for 21 days. After, was analyzed the effects on ovicidal activity: effect 1 (accession shell); effect 2 (penetration hyphae); and effect 3 (destruction of the eggs). No differences were observed in the destruction of eggs between the two isolates. The decreasing effectiveness of the different culture media was: PDA (38.9%); CMA (38.3%); WA (36.7%); YPSSA (36.45%); and AC (32.5%). The highest percentage egg destruction (effect 3) was observed when the strains were grown in culture medium AELA (44.9%); this was the best medium. The results demonstrate the effectiveness of the ovicidal helmintophagous fungus P. chlamydosporia grown in supplemented culture media in the destruction of P. equorum eggs.
