MORPHOLOGICAL CHANGES IN SHEEP NEMATODES BY CYMBOPOGON CITRATUS AND ALLIUM SATIVUM CRUDE METHANOLIC EXTRACT
Autor(es): Letícia Oliveira da Rocha, Bruna da Silva , Adriele Alvarenga , Glória Cristina da Silva Lemos , Silvério de Paiva Freitas, Ivo José Curcino Vieira , Clóvis de Paula Santos
MORPHOLOGICAL CHANGES IN SHEEP NEMATODES BY CYMBOPOGON CITRATUS AND ALLIUM SATIVUM CRUDE METHANOLIC EXTRACT
» Área de pesquisa: HELMINTOLOGIA
» Instituição: Universidade Estadual do Norte Fluminense Darcy Ribeiro
» Agência de fomento e patrocinadores: CAPES, FAPERJ, UENF
Phytotherapy is one of the promising alternatives of the control of gastrointestinal nematodes researched in recent years whose focus basically concentrates to determine the activity of plant extracts and active principles involved without however evaluate the effect on the parasite. This study evaluated the effect of the extracts of Cymbopogon citrates and Allium sativum on the morphology of infective larvae (L3) of gastrointestinal nematodes of sheep. The L3 were incubated with methanolic crude extracts of A. sativum and C. citratus at concentrations of 1.56; 6.25 and 25.00mg/ml. After 48h of incubation the samples were processed for scanning (SEM) and transmission electron microscopy (TEM). The samples were fixed in 2.5% glutaraldehyde, 4% freshly prepared formaldehyde and 5 mM of calcium chloride in cacodylate buffer, pH 7.2, and post-fixed with 1% osmium tetroxide, 5 mM calcium chloride and 0.8% potassium ferrocyanide in cacodylate buffer. The samples were dehydrated in an alcohol series, critical point dried, sputter-coated with gold and examined using a Zeiss DSM 962 SEM or samples were dehydrated in an acetone series, infiltrated in resin Spurr, polymerised in an oven at 60, sliced of 0,5μm or 70 nm thick were obtained and stained with 1% toluidine blue or contrasted in uranyl acetate and lead citrate, respectively, and were examined using an Axioplan microscopy or a Zeiss 900 TEM. Deformations of the cuticle and wilting along the body of the nematodes at all concentrations were seen. The sheath showed higher contrast in treated larvae. Apparently there was a change in the morphology of the pattern of arrangement of cuticular layers and sheath in some regions of the body. A large number of vacuoles were visualized within the intestinal tract and a slight decoupling of the hypodermis in relation to the cuticle. The analysis of changes in eggs is on its way.
