MOLECULAR DETECTION OF HEMOTROPHIC MYCOPLASMAS AMONG DOMICILED AND FREE-ROAMING CATS IN CAMPO GRANDE, MS
Autor(es): Marcos Rogério André, Heitor Miraglia Herrera, Ana Cláudia Gabriela Alexandre de Santis, Keyla Cartens Marques de Sousa, Luiz Ricardo Gonçalves, Nathani Cristina Baccarim Denardi, Iara Helena Domingos, João Bosco Vilela Campos, Rosangela Zacarias Machado
MOLECULAR DETECTION OF HEMOTROPHIC MYCOPLASMAS AMONG DOMICILED AND FREE-ROAMING CATS IN CAMPO GRANDE, MS
» Área de pesquisa: DOENÇAS VETORIAIS
» Instituição: Universidade Estadual Paulista (FCAV-UNESP Jaboticabal)
» Agência de fomento e patrocinadores: Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) providing scholarship (2013/06016-6) and financial support (2013/09889-0)
Pró-Reitoria de Pesquisa da UNESP Processo 1939/009/13-PROPe/CDC
Hemoplasmas are bacteria found attached to the feline red blood cells. The present work aimed to detect the presence of hemoplasmas in domiciled and free-roaming cats in Campo Grande, state of Mato Grosso do Sul (MS), Brazil, by molecular techniques. Between January/2013 and April/2013, EDTA-whole blood samples were collected from 151 domestic cats (65 free-roaming and 86 domiciled cats; 53 males, 95 females, and two without gender registration). Sampled feline blood samples were submitted to PCR assays targeting 16S rRNA-hemoplasmas, followed by sequencing, BLAST analysis and phylogenetic inferences. Twenty-three (15.2%) cats were positive for 'Candidatus Mycoplasma haemominutum', 17 (11.2%) for M. haemofelis, and 15 (9.9%) for 'Candidatus M. turicensis' based on 16S rRNA PCRs. Coinfection by two or three hemoplasmas were found in 25 (16.6%) cats. Related to positivity in PCR assays for hemoplasma, no statistically significant difference between gender and life-style of the cats was observed. The present work showed that different species of hemoplasmas circulate among cats population (domiciled and stray cats) in Campo Grande, MS, Brazil. Differential diagnosis with feline hemoplasmosis is suggested when cats showing non-specific clinical signs of disease manifestation.
